pulse-field electrophoresis

pulse-field electrophoresis
A method used for high-resolution electrophoretic separation of very large (megabase) fragments of DNA. Electric fields 100° apart (the angle may vary) are applied to the separation gel alternately. The continuous change of direction prevents the molecules aligning in the electric field and greatly improves resolution on the axis between the two fields.

Dictionary of molecular biology. 2004.

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