pulse-field electrophoresis

pulse-field electrophoresis
A method used for high-resolution electrophoretic separation of very large (megabase) fragments of DNA. Electric fields 100° apart (the angle may vary) are applied to the separation gel alternately. The continuous change of direction prevents the molecules aligning in the electric field and greatly improves resolution on the axis between the two fields.

Dictionary of molecular biology. 2004.

Игры ⚽ Поможем сделать НИР

Look at other dictionaries:

  • electrophoresis — Separation of molecules based on their mobility in an electric field. High resolution techniques normally use a gel support for the fluid phase. Examples of gels used are starch, acrylamide, agarose or mixtures of acrylamide and agarose.… …   Dictionary of molecular biology

  • Electrophoresis — Method used in clinical and research laboratories for separating molecules according their size and electrical charge. Electrophoresis is used to separate large molecules (such as DNA fragments or proteins) from a mixture of molecules. An… …   Medical dictionary

  • Pulsed field gel electrophoresis — Historical Background Standard gel electrophoresis techniques for separation of DNA molecules provided huge advantages for molecular biology research. However, many limitations existed with the standard protocol in that it was unable to separate… …   Wikipedia

  • Charles Cantor — Born 1942 Fields Molecular genetics Known for …   Wikipedia

  • Clostridium difficile — C. difficile colonies on a blood agar plate …   Wikipedia

  • Mycoplasma laboratorium — is a planned partially synthetic species of bacterium derived from the genome of Mycoplasma genitalium. This effort in synthetic biology is being undertaken at the J. Craig Venter Institute by a team of approximately 20 scientists headed by Nobel …   Wikipedia

  • List of electronics topics — Alphabetization has been neglected in some parts of this article (the b section in particular). You can help by editing it. This is a list of communications, computers, electronic circuits, fiberoptics, microelectronics, medical electronics,… …   Wikipedia

  • Microfluidics — deals with the behavior, precise control and manipulation of fluids that are geometrically constrained to a small, typically sub millimeter, scale. Typically, micro means one of the following features: small volumes (nl, pl, fl) small size low… …   Wikipedia

  • Common chimpanzee — Common chimpanzee[1] Conservation status …   Wikipedia

  • List of molecular biology topics — This is a list of topics in molecular biology. See also list of biochemistry topics. NOTOC # 3 end 3 flanking region 5 end 5 flanking region 5 ribose 3 A acrylamide gels adenine adenosine deaminase deficiency adenovirus agarose gel… …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”